Shantani
Drug Target Identification, Validation and MOA Studies Services
We offer application of established and advanced chemical proteomics methodologies to identify targets of bioactive small and large molecules. In tandem we combine chemical biology, biophysics and cell & molecular biology methods to validate the targets and to generate comprehensive information on the action mechanism of the molecules.
To best suit the customer's drug development needs we offer different levels of target identification services. For the detailed workflow at each level click the corresponding links below. What we need from customer is just the structure of the bait small molecule (in case of large molecule, the actual molecule) and the description of the appropriate biological system.
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Target ID Method Levels |
Key Points of Considerations |
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Fast and inexpensive, Good for comparative data analysis if the target id data is available from other methodologies, also good for generating crude information about the proteome to which the bait molecule interacts |
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More specific target information then level 1, Fast, may be utilized as stand-alone method for target identification |
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Shantani's advanced technology, cellular location specific target capture from live cells, in-depth target information then level 3, can be utilized as stand-alone method for target identification, allows clear Go/ No Go decision making |
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Target ID Method
Levels |
Typical Numbers of Deconvoluted Targets (Based on the concept of specificity ratio Saxena et al . 2008) |
Quantitative Analysis of Small-molecule target Interaction |
Target Validation in Cellular Models |
Action Mechanism Studies |
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Level 1 |
~ 25-30 |
None (unless specified) |
None (unless specified) |
None |
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Level 2
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~12-18 |
None (unless specified) |
Need Based |
'in-silico' studies |
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Level 3 |
~4-8 |
For almost all identified targets |
For almost all identified targets |
'in-silico' studies + wet lab validation |
